Loureiro, J. R.; Castro, A. F.; Figueiredo, A. S.; Eufrasio, A.; Dhingra, A.; Galhardo, M.; Marcelino, H.; Rodrigues, C.; Sampaio, P.; Azevedo, M. M.; Sousa, M.; Doria, S.; Rizzu, P.; Heutink, P.; Bessa, J.; Silveira, I.

Alu are evolutionarily very old primate-specific interspersed repeat elements that constitute ~11% of the human genome. They are a source of short tandem repeats (STRs), which often expand in size and originate inherited neuromuscular and neurodegenerative disorders. How expanded STR insertion mutations within Alu STRs culminate in disease remains unknown. Here we report an Alu STR located in an intron of DAB1 that functions as a neurodevelopmental enhancer. We demonstrated that an ATTTC repeat insertion in this DAB1 Alu STR, known to cause spinocerebellar ataxia type 37 (SCA37), hyperactivates a neurodevelopmental DAB1 enhancer. Importantly, we showed that neurons derived from SCA37 subjects have higher levels of DAB1 expression and DAB1 overexpression causes abnormal axonal pathfinding in vivo. Overall, these results establish that neuronal dysregulation of a developmental DAB1 Alu STR enhancer contributes to SCA37 pathogenesis, an unexplored mechanism likely acting in many Alu STR diseases, potentially reshaping the therapeutic landscape.