Parajuli, B.; Midya, V.; Kiddle, R.; De Jager, N.; Eggers, S.; Spakowicz, D.; Hoyd, R.; Salhia, B.; Chan, C. H.; Churchman, M.; Rounbehler, R. J.; Yao, S.; Rutkowski, M. R.; Tarhini, A. A.; Mudaranthakam, D. P.; Masood, A.; Bocklage, T. J.; Lentz, R. W.; Hatoum, H.; Ilozumba, M. N.; Hardikar, S.; Ulrich, C. M.; Round, J. L.; Riedlinger, G.; Shriver, C. D.; Bosch, D. E.

Metastasis causes most cancer-related deaths in colorectal carcinoma (CRC), and microbiome markers may have prognostic value. We hypothesized that primary tumor microbiomes predict distant metastases. We analyzed 5-year metastasis-free survival (MFS) in a retrospective cohort of 900 ORIEN CRC tumor microbiomes (RNAseq). ORIEN findings were validated on an independent cohort using 16S rDNA sequencing and pathobiont-specific qPCR. Microbiome alpha diversity was higher in primary tumors than metastases and positively correlated with metastasis risk. Microbiome beta diversity distinguished primary vs. metastasis and predicted 5-year MFS. High primary tumor abundance of B. fragilis and low F. nucleatum were associated with short MFS. Enterobacteriaceae, including E. coli, were enriched in metastases. qPCR identified increased enterotoxigenic B. fragilis and pks+ E. coli detection in CRC metastasizers. Microbial co-occurrence analysis identified a 3-species clique that predicts metastasis (OR 1.9 [1.4-2.6]). Results suggest that primary tumor microbiomes and specific pathobionts are precision markers for metastasis risk.